Contents
- 1 What does it mean when a gene is highly expressed?
- 2 What is the acceptable coefficient of variation?
- 3 How many genes are expressed?
- 4 How to calculate per batch coefficient of variation?
- 5 What is the coefficient of variation after adjustment?
- 6 Which is higher the coefficient of variation or the GCV?
What does it mean when a gene is highly expressed?
A gene is predicted highly expressed (PHX) if its codon frequencies are close to those of the ribosomal proteins, major translation/transcription processing factor, and chaperone/degradation standards but strongly deviant from the average gene codon frequencies.
What is the acceptable coefficient of variation?
Basically CV<10 is very good, 10-20 is good, 20-30 is acceptable, and CV>30 is not acceptable.
How many genes are expressed?
The number of genes expressed in a tissue ranged from 11,199 to 15,518 genes (Table 2), so a majority of the genes expressed in a specific tissue or cell type are ubiquitously expressed genes.
What happens during gene expression?
Gene expression is the process by which the instructions in our DNA are converted into a functional product, such as a protein. It acts as both an on/off switch to control when proteins are made and also a volume control that increases or decreases the amount of proteins made.
How to compare coefficients of variation in gene expression?
We would like to compare coefficients of variation of gene expression across individuals, a metric that is widely employed for quantifying heterogeneity of gene expression in single cell sequencing data.
How to calculate per batch coefficient of variation?
Compute per batch coefficient of variation based on transformed molecule counts (on count scale). Include only genes with positive coefficient of variation. Some genes in this data may have zero coefficient of variation, because we include gene with more than 0 count across all cells.
What is the coefficient of variation after adjustment?
All genes: coefficient of variation after adjustment. All genes: coefficient of variation before adjustment. Endogeneous genes: coefficient of variation after adjustment. Endogeneous genes: coefficient of variation before adjustment.
Which is higher the coefficient of variation or the GCV?
In general, the magnitude of phenotypic coefficient of variation (PCV) was higher than the corresponding genotypic coefficient of variation (GCV) for all the traits.