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Can a gene have multiple transcripts?
However, a single gene can produce multiple transcripts and these can be very different one from the other (see for example the table of transcripts of the Actin gene, there are several coding and non coding transcripts).
Why do genes have multiple transcripts?
As for finding multiple transcripts for a given gene, this is the result of alternative splicing, which is part of what contributes to making an organism (such as ourselves) so complex with a relatively small number of genes. And Daniel is right – every alternative transcript has a biological function.
Does more genes mean more complex?
Accordingly, one might expect that: «more complex organisms have larger genomes and contain a larger number of genes». That is, throughout evolution an increase in genome sizes and the number of genes is expected….Number of genes and complexity of the organism.
| Organism | Eukaryotes |
|---|---|
| Saccharomyces cerevisiae | |
| 12 | |
| 6,241 | |
| 480 |
What is the difference between the gene and the transcript?
Transcripts are defined as RNA molecules that are made from a DNA template. Most genes have multiple transcripts associated with them. Some of these transcripts differ only in the “untranslated regions” (UTRs), while the coding sequence and resulting protein stay the same.
How is StringTie used in the transcriptome?
Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts.
What kind of gene transfer format does StringTie use?
The primary output of StringTie is a Gene Transfer Format (GTF) file that contains details of the transcripts that StringTie assembles from RNA-Seq data. GTF is an extension of GFF (Gene Finding Format, also called General Feature Format), and is very similar to GFF2 and GFF3.
How is StringTie used in RNA Seq alignment?
StringTie is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length transcripts representing multiple splice variants for each gene locus.
How are gene abundances reported in StringTie file?
Gene abundances will be reported (tab delimited format) in the output file with the given name. StringTie outputs a file with the given name with all transcripts in the provided reference file that are fully covered by reads (requires -G ).