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Why are biological replicates important?
[3] Biological replicates are important because they address how widely your experimental results can be generalized. They indicate if an experimental effect is sustainable under a different set of biological variables.
Where are biological replicates used?
Biological replicates are different samples measured across multiple conditions, e.g., six different human samples across six arrays. Using replicates offers three major advantages: Replicates can be used to measure variation in the experiment so that statistical tests can be applied to evaluate differences.
Why do you need to run replicates for each sample?
You must run the samples in triplicates too to make sure your results are statistically valid and not artifacts. Doubling dilutions for standards can be done in duplicate if necessary because the other dilutions will make an outlier obvious and it can be discarded.
What is an example of replication in biology?
In prokaryotes, with a small, simple, circular chromosome, only one origin of replication is needed to replicate the whole genome. For example, E. coli has a ~4.5 Mb genome (chromosome) that can be duplicated in ~40 minutes assuming a single origin, bi-directional replication, and a speed of ~1000 bases/second/fork for the polymerase.
What is the definition of replication in biology?
Replicate (biology) In the biological sciences, a replicate is an exact copy of a sample that is being analyzed, such as a cell, organism or molecule, on which exactly the same procedure is done. This is often done in order to check for experimental or procedural error.
Is DNA replication always foolproof process?
Unfortunately, DNA replication is not a fool-proof process. For various reasons, errors can crop up. Sometimes these errors don’t make any difference in the way the genome functions, and sometimes the errors can be repaired. In some cases, however, a replication error can give rise to a genetic disease.
Does DNA replication always start at origin of replication?
An origin of replication is the specific site in DNA where replication begins; while there is only one origin of replication in circular prokaryotic DNA, linear eukaryotic DNA has many origins of replication. Replication always starts at specific locations in DNA, which are called origins of replication.