Contents
How does RNA-Seq compare to microarray?
The main difference between RNA-Seq and microarrays is that the former allows for full sequencing of the whole transcriptome while the latter only profiles predefined transcripts/genes through hybridization.
What is an advantage of RNA-Seq over microarray?
The advantage of RNA-Seq over microarrays is that it provides an unbiased insight into all transcripts (Zhao et al., 2014). Thus, RNA-Seq is generally reliable for accurately measuring gene expression level changes.
What is an expression microarray?
A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. The DNA molecules attached to each slide act as probes to detect gene expression, which is also known as the transcriptome or the set of messenger RNA (mRNA) transcripts expressed by a group of genes.
Can Microarray be used for RNA?
DNA microarrays can be used to detect DNA (as in comparative genomic hybridization), or detect RNA (most commonly as cDNA after reverse transcription) that may or may not be translated into proteins.
Is RNA or microarray faster?
“mRNA-Seq offers improved specificity, so it’s better at detecting transcripts, and specifically isoforms, than microarrays. It’s also more sensitive in detecting differential expression and offers increased dynamic range.”
What is the difference between microarray and next generation sequencing?
Microarray analysis is limited only by the preparation of sufficient target DNA samples and probe-spotted microarray slides; in contrast, NGS analysis is limited by the number of samples processed in a single run by the physical partitioning or sample-specific barcoding approach utilized.
Are microarrays still used?
Today, DNA microarrays are used in clinical diagnostic tests for some diseases. Sometimes they are also used to determine which drugs might be best prescribed for particular individuals, because genes determine how our bodies handle the chemistry related to those drugs.
Which is the first step in using a DNA microarray?
The first step in using a microarray is to collect healthy and cancerous tissue samples from the patient. This way, doctors can look at what genes are turned on and off in the healthy cells compared to the cancerous cells. Once the tissues samples are obtained, the messenger RNA (mRNA) is isolated from the samples.
When would you use a microarray?
Microarrays can also be used to study the extent to which certain genes are turned on or off in cells and tissues. In this case, instead of isolating DNA from the samples, RNA (which is a transcript of the DNA) is isolated and measured. Today, DNA microarrays are used in clinical diagnostic tests for some diseases.
What can microarray not detect?
There are several limitations to chromosomal microarray. For example, microarray analysis cannot detect balanced chromosome rearrangements (eg, inversions or translocations), which do not result in deletion or duplication of genetic material, or cases of low-level tissue mosaicism.