Contents
Why remove duplicate reads?
In our RNA-Seq data we tend to get very uneven coverage of expressed genes. Removing duplicates would eliminate much of our signal from those genes, and all our read counts would be dramatically lower.
How to remove PCR duplicates?
PCR duplicates are reads that are made from the same original cDNA molecule via PCR. A common practice to eliminate PCR duplicates is to remove all but one read of identical sequences, assuming that such reads have been created from the same cDNA molecule by PCR [3].
Does PCR duplicate the entire genome?
PCR duplicates arise in step 3. In step 3 you are intentionally creating multiple copies of each original genomic DNA molecule so that you have enough of them, therefore some amount of PCR duplication is inevitable.
What is a PCR duplicate?
PCR duplicates are sequence reads that result from sequencing two or more copies of the exact same DNA fragment, which, at worst, may contain erroneous mutations introduced during PCR amplification, or, at the very least, make the occurrence of the allele(s) sequenced in duplicates appear proportionately more often …
Are there any free programs to remove duplicate files?
Duplicate Cleaner Free. Duplicate Cleaner by DigitalVolcano Software is the leading program for finding and removing duplicate files on your Windows PC. Documents, pictures, music and more – this app will find it all. This free version has a subset of features found in it’s big brother, Duplicate Cleaner Pro.
How can I get rid of duplicates in a spreadsheet?
Open a new spreadsheet in Google Docs or use an existing spreadsheet containing duplicates. If the spreadsheet is empty, add a few rows of data (for example, a list of contacts, parts inventory, etc.) and duplicate some of them.
How can I duplicate data in Google Docs?
Running a simple example. Open a new spreadsheet in Google Docs or use an existing spreadsheet containing duplicates. If the spreadsheet is empty, add a few rows of data (for example, a list of contacts, parts inventory, etc.) and duplicate some of them. Choose the menu Tools > Script Editor.
Is it possible to avoid duplicates in Apps Script?
You should now be able to avoid duplicates when manipulating data with Apps Script. Except as otherwise noted, the content of this page is licensed under the Creative Commons Attribution 4.0 License, and code samples are licensed under the Apache 2.0 License. For details, see the Google Developers Site Policies.